Current Issue : January - March Volume : 2021 Issue Number : 1 Articles : 5 Articles
The programmed cell death protein-1 (PD-1)/programmed cell death ligand-1 (PD-L1) axis\nblockade has been implemented in advanced-stage tumor therapy for various entities, including head\nand neck squamous cell carcinoma (HNSCC). Despite a promising tumor response in a subgroup of\nHNSCC patients, the majority suffer from disease progression. PD-L1 is known to influence several\nintrinsic mechanisms in cancer cells, such as proliferation, apoptosis, migration and invasion. Here,\nwe modulated PD-L1 expression in three HNSCC cell lines with differential intrinsic PD-L1 expression.\nIn addition to an alteration in the epithelial-to-mesenchymal transition (EMT) marker expression,\nwe observed PD-L1-dependent cell spreading, migration and invasion in a spheroid spreading assay\non four different coatings (poly-L-lysine, collagen type I, fibronectin and Matrigel®) and a chemotactic\ntranswell migration/invasion assay. Furthermore, the overexpression of PD-L1 led to increased gene\nexpression and small interfering ribonucleic acid (siRNA) knockdown and decreased gene expression\nof Rho-GTPases and related proteins.....................
Orofacial cleft (OFC) is one of the most prevalent birth defects, leading to substantial and\nlong-term burdens in a newbornâ??s quality of life. Although studies revealed several genetic variants\nassociated with the birth defect, novel approaches may provide additional clues about its etiology.\nUsing the Center for Craniofacial and Dental Genetics project data (n = 10,542), we performed linear\nmixed-model analyses to study the genetic compositions of OFC and investigated the dependence\namong identified loci using conditional analyses. To identify genes associated with OFC, we conducted\na transcriptome-wide association study (TWAS) based on predicted expression levels. In addition to\nconfirming the previous findings at four loci, 1q32.2, 8q24, 2p24.2 and 17p13.1, we untwined two\nindependent loci at 1q32.2, TRAF3IP3 and IRF6..................................
Background: Erythropoietin has a pivotal role in erythropoiesis and angiogenesis.\nA common polymorphism...........................
Baculovirus expression systems have been widely used to produce recombinant mammalian\nproteins owing to the lack of viral replication in vertebrates. Although several lines of evidence\nhave demonstrated impacts of baculovirus infection in mammalian hosts, genome-wide effects have\nnot been fully elucidated. Here, we provide comparative transcriptome profiles of baculovirus\nand host-immune response genes in recombinant baculovirus-infected mammalian and insect cells.\nSpecifically, to decipher the impacts of baculovirus infection in mammalian cells, we conducted total\nRNA-seq on human 293TT cells and insect Sf9 cells infected with recombinant baculovirus. We found\nthat baculovirus genes were rarely expressed under the control of baculoviral promoters in 293TT\ncells. Although some baculovirus early genes, such as PE38 and IE-01, showed limited expression in\n293TT cells, baculoviral late genes were mostly silent. We also found modest induction of a small\nnumber of mammalian immune response genes associated with Toll-like receptors, cytokine signaling,\nand complement in baculovirus-infected 293TT cells. These comprehensive transcriptome data\nwill contribute to improving recombinant baculovirus as tools for gene delivery, gene therapy,\nand vaccine development....
Previously, we used a lentiviral vector to deliver furin-cleavable human insulin (INS-FUR)\nto the livers in several animal models of diabetes using intervallic infusion in full flow occlusion (FFO),\nwith resultant reversal of diabetes, restoration of glucose tolerance and pancreatic transdifferentiation\n(PT), due to the expression of beta...............................
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